Last Update 30/ 05/ 2000
in English/ in Esperanto/ in French/ in Portuguese
CHROMATOGRAPHY: several techniques are utilized in this method. Its main objective is to separate substances from a mixture, either with analytical or preparative purposes. It is frequently used in industrial, research and educational laboratories. All the chromatographic techniques utilize a stationary phase and a mobile phase. The stationary phase is formed by a selected material in order to differently retain the components to be separated from the sample. After the migration through the stationary phase for a conveniently selected distance, the components of the sample are separated and accused by the detection system in the sequence: from the first component, less retained, to the last component, more retained, by the stationary phase. The model shown by the above animation represents a chromatographic separation of the letters in the word "cromatografia" (which means chromatography in Portuguese) introduced in a stationary phase in the syringe, where the less retained letter is the first in the alphabet, and the more retained the last. The other letters are retained inside these limits, in alphabetic order. The black coloured diagram is named chromatogram. It represents the peak heights as well, simulating the concentration of the component in the injected sample. The peak height is proportional to the quantity of equal letters in the word.
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